Chronoamperometric Study of Bisulfite Reduction by Myoglobin
نویسنده
چکیده
The kinetics of the reduction of bisulfite by surfactant myoglobin films were examined using chronoamperometry after the overall stoichiometry of the reduction was examined by coulometry. Surfactant films of myoglobin were formed on a graphite (HOPG) electrode. On this electrode, two waves were observed for myoglobin, corresponding to the Fe(III)/Fe(II) and Fe(II)/Fe(I) reductions. Cyclic voltammetry had demonstrated that no catalytic current for bisulfite reduction was observed for the first wave, while significant catalysis was observed for the second wave. Coulometric studies verified that the overall reaction was a 6-electron reduction to hydrogen sulfide. In order to separate electron transfer kinetics from the kinetics of the reduction of bisulfite by myoglobin, chronoamperometry was used. If the electron transfer rate was fast compared to the bisulfite reduction kinetics (which can be assured by choosing a potential well negative of the peak potential), the catalytic current is given by:
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